The roles of CRISPR-Cas9 and RNA interference mechanisms in increasing yield in agriculture and struggle insects

Abstract :

In recent years, the CRISPR / Cas9 (regularly sequenced short palindromic repeats and associated protein) mechanism, which has become a genome editing tool using single guided RNAs, is a new generation gene editing technique that allows genetic changes in plants. This system, which initially formed the acquired immunity in bacteria, has made rapid progress from immune response to genome editing systems as a genome engineering tool. The CRISPR-Cas9 mechanism, especially the simple and high efficiency of extracting, cutting and editing DNA sequences, makes it advantageous for this system to target the target region in the genome sequences. Apart from this, the RNA Interference (RNAi) mechanism also reduces insect damage or insect population by silencing foreign target gene parts. Thus, CRISPR-Cas9 and RNAi systems, which are one of their fields of study, used in agriculture, are being studied as systems that improve the target genes in crops and also increase production and yield by combating insects that damage the crop, and produce plants resistant to disease resistance. In our article, we will transfer CRISPR-Cas9 and RNAi systems in this context.


Keyword :

CRISPR-Cas9, RNAi, genome editing, agriculture, crop, insect


Author(s) : Kantar, B., Taştan, H.
Downloads : 592
Published Issue : 2021 Vol. 16 Number 2


2021 Vol. 16 Number 2