The hemocyte types, differential and total count in Papilio demoleus L. (Lepidoptera: Papilionidae) during post-embryonic development

Abstract :

The present study was undertaken to recognize the types of hemocytes of P. demoleus by electron microscopy. Six types were identified: prohemocytes (PR), plasmatocytes (PL), granulocytes (GR), spherulocytes (SP), adipohemocytes (AD), oenocytoids (OE), with two additional types, vermicytes (VE) and podocytes (PO), having debatable identities. The PRs were mostly rounded small cells with characteristically large and filling nucleus showing an uneven surface due to the presence of vaguely concentric and elevated circles by SEM. The PLs were polymorphic but largely spindle–shaped. The GRs were rounded to ovoid with relatively small and eccentric nucleus. The SPs were also rounded to ovoid with cytoplasm appearing lobulated. The ADs were rounded to elongate with cytoplasm containing lipid droplets. The OEs were rounded, and mostly large. The PL and GR remained relatively high and SP, AD and OE low throughout the postembryonic development. The VEs, were greatly elongated and narrow cells with pointed ends. The POs were flat cells with three or more cytoplasmic arms projecting from a central body. These later cells appear for a limited period only in the early pupa. The THC steadily decreases during the developmental stages, attains its peak in the late 5th (prepupa) instar and steeply declines in the pupa.

Keyword :

Hemocytes, morphology, total hemocyte count, differential hemocyte count, Papilio demoleus

Author(s) : Jalali, J., Salehi, R.
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Published Issue : 2008 Vol. 3 Number 1

2008 Vol. 3 Number 1