Review of CRISPR-Cas9 studies in the field of Entomology

Abstract :

The CRISPR-Cas9 system consists of periodically clustered short palindromic repeats (CRISPR) and the CRISPR-associated Cas9 component. This technique is an easily adaptable genome editing technology that can reach many regions on the genome. This technology is frequently preferred because it precisely makes targeted genetic changes in various species. Compared to zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and RNA interference (RNAi), the CRISPR-Cas9 system has the advantage of obtaining precise and meaningful data in a shorter time. This method has been applied to many insect species in genome editing studies in the field of entomology. In this article, the genome modifications mediated by CRISPR-Cas9 on different insect species and the benefits of these modifications, by making a comprehensive literature review, the data obtained are clearly conveyed. Thus, it is aimed to inform the broad masses, especially biologists, about the use of the CRISPR-Cas9 system, which is one of the popular techniques, in entomology.

Keyword :

CRISPR-Cas9, gene editing, insects

Author(s) : Uşak, B., Taştan, H.
Downloads : 632
Published Issue : 2021 Vol. 16 Number 2

2021 Vol. 16 Number 2