Partial characterization of glutathione s-transferase in two populations of the sunn pest, Eurygaster integriceps Puton (Heteroptera: Scutellaridae)

Abstract :

Glutathione S-transferases (GSTs) from two populations (Tehran and Shiraz) of the sunn pest Eurygaster integriceps Puton (Heteroptera: Scutellaridae) were characterized through in vitro colorimetric assays. GSTs showed higher activity peaks at pH 5-6. The Km-values for GSTs were different between populations in which Shiraz population had higher Vmax and lower Km values when using 1-chloro-2,4-dinitrobenzene (CDNB) as substrate (and a fixed concentration of reduced glutathione—GSH). In addition, Native-PAGE electrophoresis showed a higher isoenzymatic patterns for Shiraz population compared with Tehran one. These results provide evidence of the involvement of enhanced GST activity as an additional organophosphorus-resistant mechanism in at least some sunn pest populations from Iran

Keyword :

Metabolic detoxification, Enhanced glutathione conjugation, fenitrothione resistance

Author(s) : Zibaee, I., Bandani, A. R., Haghani, S., Zibaee, A.
Downloads : 2757
Published Issue : 2009 Vol. 4 Number 2

2009 Vol. 4 Number 2