22 Eyl 2025 Pazartesi
Optimizing developmental periods of Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) via response surface methodology
Abstract : This study was conducted to investigate the pre-adult developmental periods of brown marmorated stink bug Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) individuals fed with different diets. The time to maturity of H. halys individuals fed with carrots, soybeans, and apricots was evaluated using the response surface methodology (RSM). In the study, adult individuals were collected from hazelnut fields in the Black Sea Region and produced under laboratory conditions to obtain F1, F2 and F3 generations. Nymphs hatched from eggs belonging to the F3 generation were fed only with carrots, soybeans, and apricots and their developmental processes were followed until adulthood. The experiments were carried out in climate cabinets with 30±1 °C temperature, 65±10 % relative humidity and 16/8 h light/dark cycle. The water needs of the individuals in the culture containers were met by filter paper and the environmental conditions were checked regularly. Eggs were collected daily to prevent any damage, and the nymphs that hatched were monitored until they became adults. The hatching time, nymphal stages, adulthood period, and reproductive stages of individuals fed with three different food groups were monitored. Using central composite design (CCD) within the scope of the RSM, the factors affecting the developmental period of H. halys were examined and combinations at different levels were tested. A response surface model was created using the experimental data and the optimum conditions that would allow the insects to reach adulthood in the fastest or desired time were determined. This study reveals that the developmental periods of H. halys change depending on the food preferences and provides important information about the biology of the invasive insect. The data obtained constitute an important scientific basis for the fight against H. halys and understanding its population dynamics.