New approach toward á-amylase electrophoresis and isoamylase detection

Abstract :

Native polyacrylamide gel electrophoresis has been a useful method for analysis of á-amylases and their isozyme(s). Routinely, for detection of enzyme activity soluble starch (1%) is added at the end of electrophoresis, before staining the gel, which is time consuming and in some cases minor isozyme(s) could not be detected. Thus, we developed a convenient and efficient native page by adding the soluble 0.5% starch at the beginning of electrophoresis as a part of gel components and running at 4 °C. This simple modification should be beneficial for analyzing the both purified and unpurified

Keyword :

á-amylase electrophoresis, isoamylase detection

Author(s) : Mehrabadi, M., Bandani, A. R.
Downloads : 2120
Published Issue : 2010 Vol. 5 Suppl.

2010 Vol. 5 Suppl.