Isolation and identification native Bacillus thuringiensis in different habitat from West Azerbaijan and evaluate effects on Indian moth Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae)

Abstract :

The bacterium Bacillus thuringiensis is characterized by the production crystalline parasporal inclusion in stationary phase in sporulation growth stage with insecticide activity. This bacterium can be isolated and identified from different environment with navel toxin and can be evaluated toxicity against different host. In this report, 48 native strains were isolated from 740 samples by acetate selection method. All isolates were characterized by crystal morphology and toxicity against 2nd instars Indian moth (Plodia interpunctella) larvae. Majority of strains (58%) had bipyramidal crystals. 16 isolates had percentage mortality more than 75% and 11 Isolates namely wz-105, wz-111, wz-120, wz-122, wz-125, wz-149, wz-155, wz-157, wz-184, wz-187 and wz-189 had percentage mortality (>90%) equal or more than B. thuringiensis kurstaki as positive control

Keyword :

B. thuringiensis, Isolates, Plodia interpunctella, toxicity

Author(s) : Aramideh, S., Saferalizadeh, M. H., Pourmirza, A. A., Bari, M. R., Keshavarzi, M., Mohseniazar, M.
Downloads : 2257
Published Issue : 2010 Vol. 5 Suppl.

2010 Vol. 5 Suppl.