In vitro anthelmintic activity of orange peel (Citrus sinensis) extract: Investigating waste valorization as a novel anthelmintic strategy

Abstract : This study aimed to evaluate the anthelmintic activity of orange peel (Citrus sinensis) extract in vitro. A series of experiments have been undertaken to demonstrate the potential anthelmintic activity of orange peel aqueous and ethanolic extract. Percentage worm motility inhibition (%WMI) was determined to assess the effect on the worm motility and mortality. The ethanolic extract of orange peel demonstrated notable efficacy, resulting in paralysis and mortality, albeit at a slightly slower rate than fenbendazole. In contrast, the aqueous extract exhibited reduced effectiveness, as evidenced by lower mortality rates and a slower response. Specifically, fenbendazole achieved 100% mortality within 2.5 hours, while 95% of helminths in the negative control group (Phosphate buffer saline) survived after six hours. Furthermore, the ethanolic orange peel extract displayed a dose-dependent effect, with the 10% concentration resulting in 100% mortality, in contrast to only 20% at a concentration of 0.31%. The efficacy of orange peel extract was demonstrated to be dose-dependent, with higher concentrations leading to more significant reductions in worm motility. This finding highlights the necessity of identifying the optimal concentration for safe application in live poultry, as excessively high doses may pose risks of side effects or toxicity.
Keyword : Poultry, anthelmintic, orange peel extract, waste valorization, In vitro
Author(s) : Iqbal, S., Abdullah, U., Hamid, T., Tanveer, S.
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Published Issue : 2025 Vol. 20 Supplement


2025 Vol. 20 Supplement