04 Haz 2025 Çarşamba
Gas chromatography-mass spectroscopy analysis and histopathological effects of methanol leaf extract of Lasianthera africana P. Beauv. in the midgut of maize weevil, Sitophilus zeamais L.
Abstract : Alternative methods for pest control have been widely used to mitigate challenges arising from pesticide applications in agriculture. This study investigated Gas Chromatography-Mass Spectrum (GC-MS) in the identification of bioactive components in methanol leaf extract of Lasianthera africana and examined its impact on the midgut histopathology of maize weevil, Sitophilus zeamais. The existence of eight (8) phytochemical substances with various therapeutic actions were shown by the GC-MS analysis: Thiirane (2.0); 1,1 dimethylhydrazine (2.8); L-aspartic acid (2.6); N-methyl-3,4-methylene dioxyphenylpropan-3-amine (1.7); 1-hydroxyimino-1-(4-methylphenyl) propan-2-one (2.3); Mercaptoethanol (1.6); 1,3-bis -t- butylperoxy-phthalan (1.3); 2-amino-4-(2-methylpropenyl)-pyrimidin-5-carboxylic acid (2.5) and other minor compounds, as the main constituents. Comparing the plant extract-treated S. zeamais specimen to the control, the histological section demonstrated a mild reorganization of the respiratory, secretory, and gastrointestinal layers along with the destruction of the muscular layer. In conclusion, the midgut histology of S. zeamais may be affected by the bioactive chemicals present in L. africana, which have a variety of biological functions. Thus, farmers are encouraged to use botanicals which are practical, ecologically friendly instead of synthetic insecticides with high residual effects to preserve their stored grains.