Compounds in abdominal and metathoracic scent glands of nymphs and adults of Graphosoma lineatum (Linnaeus, 1758) (Het., Scutelleridae) under laboratory conditions

Abstract :

The contents of metathoracic glands of two sexes and different ages of adult Graphosoma lineatum (L.) were analyzed at laboratory conditions. Insect rearing were carried out on the seeds of three species of umbelliferous plants (Parsley, Petroselinum crispum; Carrot, Daucus carota; and coriander, Coriandrum sativum). The scent gland compounds of 3- 15- and 25-days old adults were extracted by solid-phase microextraction (SPME) and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). In this study, SPME and direct injection were applied to evaluate commercial and common fiber. The results showed that SPME method and common pencil fiber were effective in detection of scent gland extracts. Different components such as tridecane, 3-methyl pentanol, cis-1,2- dimethyl cyclopentane, hexane, heptane, (E)-2-hexenal, ethyl cyclohexane, limonene, (Z)-4-hexen-1-ol acetate, undecane, dodecane, (E)-2-decenal, 1-tridecene, tetradecane, (E)-5-decenyl acetate, pentadecane and hexadecane were detected in scent glands which tridecane and 3-methyl pentanol were the major components. Comparison of chromatographic peak area obtained in the same experimental conditions revealed that the abundance of some compounds varied considerably by the sex and age of insects

Keyword :

Chemical analysis, Graphosoma lineatum, Insect, Scent glands

Author(s) : Pour Abad, R. F., Azhari, S., Djozan, D., Hejazi, M. J.
Downloads : 1789
Published Issue : 2012 Vol. 7 Number 2

2012 Vol. 7 Number 2