New approach toward á-amylase electrophoresis and isoamylase detection

Abstract : Native polyacrylamide gel electrophoresis has been a useful method for analysis of á-amylases and their isozyme(s). Routinely, for detection of enzyme activity soluble starch (1%) is added at the end of electrophoresis, before staining the gel, which is time consuming and in some cases minor isozyme(s) could not be detected. Thus, we developed a convenient and efficient native page by adding the soluble 0.5% starch at the beginning of electrophoresis as a part of gel components and running at 4 °C. This simple modification should be beneficial for analyzing the both purified and unpurified
Keyword : á-amylase electrophoresis, isoamylase detection
Author(s) : , M. Mehrabadi, A. R. Bandani
Downloads : 2100

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